{"id":6063,"date":"2024-12-12T15:47:32","date_gmt":"2024-12-12T20:47:32","guid":{"rendered":"https:\/\/dkc.umw.edu\/?p=6063"},"modified":"2024-12-12T15:47:32","modified_gmt":"2024-12-12T20:47:32","slug":"bcr-abl-part-2-3d-molecular-representations","status":"publish","type":"post","link":"https:\/\/dkc.umw.edu\/fellows\/2024\/12\/12\/bcr-abl-part-2-3d-molecular-representations\/","title":{"rendered":"BCR-ABL Part 2: 3D Molecular Representations"},"content":{"rendered":"\n
\"Tony<\/figure>\n\n\n\n

In my last post I went over how I edited my BCR-ABL model in Fusion360 but here I will discuss the printing process. I have now returned to using the multi-extruder with some new tools and settings which I will discuss later on. Spoiler alert, IT WORKED!<\/p>\n\n\n\n

\"Image<\/figure>\n\n\n\n

New Filament…<\/h2>\n\n\n\n

One of the things that I was unable to do at the start of this project was use soluble filament for support structures. I had originally used a PVA filament from Polymaker which has a printing temperature of 215-225\u00b0C and costed around 60$ for a 0.75kg wheel. We have since upgraded to a BVOH filament from Verbatim which has a printing temperature of 210 \u00b1 10\u00b0C and costed upwards of 140$ for a 500g wheel. Overall, BVOH filament is far superior in all regards as it dissolves in water faster, it does not absorb humidity as fast and therefore can last longer, the possibilities of “stringiness” during printing is lower, and the extrusion can be more continuous. Now yes, it is also far more expensive, but I was completely unable to get anything to print with PVA and I made progress with BVOH. I believe this filament was much better than PVA because the printing temperature of BVOH is very similar to that of PLA meaning temperatures remain relatively constant throughout the print. This reduces the chance of “goopy” filament extrusion due to temperatures being too high and reduces the chance of filament solidification (clogging) from temperatures being too low. <\/p>\n\n\n\n

\"Image<\/figure>\n\n\n\n

Filament Dryer…<\/h2>\n\n\n\n

As I previously mentioned, BVOH filament does not absorb moisture as well as PVA, however soluble filaments across the board are more susceptible to going bad from sitting out compared to other filament types. This is most apparent during prints where the filaments can be sitting in open air for hours at a time. To resolve this issue, we purchased a COMGROW Filament dryer. This dryer can hold two wheels of filament at a time which can be left running during print times. An image is posted below.<\/p>\n\n\n\n

\"Image<\/figure>\n\n\n\n

Print Settings…<\/h2>\n\n\n\n

Now these print settings have not changed much, and the biggest difference was made by using a different filament with a filament dryer. <\/p>\n\n\n\n

\"Image<\/figure>\n\n\n\n

The main changes that I wish to highlight are all under “Options for support material and raft.” <\/p>\n\n\n\n

    \n
  • Style: Organic (read my last post for more details on organic supports)<\/li>\n\n\n\n
  • Top Contact Z distance: 0 (this is what is recommended for soluble support printing)<\/li>\n\n\n\n
  • Top Interface layer: 1 (this is just to further improve upon removal of supports)<\/li>\n\n\n\n
  • Interface pattern: concentric (this is what is recommended for soluble support printing)<\/li>\n<\/ul>\n\n\n\n
    \"Image<\/figure>\n\n\n\n

    These are the temperatures I had set for the PLA extruders. (note: bed temperature was later set to 70\u00b0C)<\/p>\n\n\n\n

    \"Image<\/figure>\n\n\n\n

    These are the temperatures I had set for the BVOH extruder. (note: bed temperature was later set to 70\u00b0C)<\/p>\n\n\n\n

    BCR-ABL Printed…<\/h2>\n\n\n\n

    This will be a small montage of the print including a video of the soluble supports submerged in water. See you one last time next week!<\/p>\n\n\n\n

    \"Shows<\/figure>\n\n\n\n

    Shows off the model fully connected together with Imatinib (red) in the substrate binding pocket. <\/p>\n\n\n\n

    \"Shows<\/figure>\n\n\n\n
    \"Image<\/figure>\n\n\n\n

    This print was a major success however there were some issues. As you can see in the second image one of the pegs broke while I was trying to join the pieces. This issue can be solved by increasing the infill to strengthen the pegs. In the third image you can see apparent warping to the pink half. This issue is something that I don’t understand yet. I assume because the model was printed peg side down there was drooping of the model despite using supports. In the final model I hope to avoid these issues by increasing infill, printing the model flipped so the peg side is up allowing for a stronger base to avoid drooping, and increasing the bed temperatures to allow the filament to stick better. <\/p>\n","protected":false},"excerpt":{"rendered":"

    In my last post I went over how I edited my BCR-ABL model in Fusion360 but here I will discuss the printing process. I have now returned to using the multi-extruder with some new tools and settings which I will discuss later on. Spoiler alert, IT WORKED! New Filament… One of the things that I […]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-6063","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/posts\/6063","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/comments?post=6063"}],"version-history":[{"count":0,"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/posts\/6063\/revisions"}],"wp:attachment":[{"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/media?parent=6063"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/categories?post=6063"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dkc.umw.edu\/fellows\/wp-json\/wp\/v2\/tags?post=6063"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}